Analysis Note

≥10 MU/mg protein

Application

Isoamylase from Pseudomonas sp. has been used in starch debranching and to measure the chain-length distributions.

Biochem/physiol Actions

Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Pseudomonas amyloderamosa is considered a popular source of isoamylase. In the presence of a surfactant, this isoamylase produces a series of maltooligosaccharides. Immobilized isoamylase makes it applicable with a polysaccharide matrix composed of agarose, cellulose, and raw corn starch.

Unit Definition

One unit causes an increase in A₆₁₀ of 0.1 in 1 hour using a homogenized solution of rice starch as a substrate (pH 3.5, 40°C)